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Identification of AFLP markers to recognize the pod spininess in okra
(Abelmoschus esculentus L.)
Muhanad Akash
Department of Horticulture and Crop Science, School of Agriculture, The
University of Jordan, Amman, Jordan
Corresponding author (e-mail: makash@ju.edu.jo)
Abstract
Association between AFLP markers and
pod spininess in 21
landrace of okra
(Abelmoschus esculentus L.)
was investigated. Six hundred AFLP bands were scored across all landraces.
The polymorphism of
AFLP bands were analyzed among landraces differing in pod
spine existence,
the significance level of frequency of band (presence and absence in the
two groups) was calculated by Fisher’s exact test. Two
reproducible AFLP bands, 187 bp and 220 bp, were present in significantly
higher frequencies in landraces with pod spininess compared with other
landraces lacking pod spines (p ≤ 0.01). These two markers were
generated from single AFLP primer combination (Mse-CAT and Eco-ACT).
The detected marker–trait associations will provide the
platform towards better understanding of the molecular
basis for pod
spine existence
in okra.
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