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International Journal of Modern Agriculture ISSN 2305-7246

 

 

 

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Identification of AFLP markers to recognize the pod spininess in okra (Abelmoschus esculentus L.)

 

Muhanad Akash

Department of Horticulture and Crop Science, School of Agriculture, The University of Jordan, Amman, Jordan

Corresponding author (e-mail: makash@ju.edu.jo)

 

Abstract

Association between AFLP markers and pod spininess in 21 landrace of okra (Abelmoschus esculentus L.) was investigated. Six hundred AFLP bands were scored across all landraces. The polymorphism of AFLP bands were analyzed among landraces differing in pod spine existence, the significance level of frequency of band (presence and absence in the two groups) was calculated by Fisher’s exact test. Two reproducible AFLP bands, 187 bp and 220 bp, were present in significantly higher frequencies in landraces with pod spininess compared with other landraces lacking pod spines (p ≤ 0.01). These two markers were generated from single AFLP primer combination (Mse-CAT and Eco-ACT). The detected marker–trait associations will provide the platform towards better understanding of the molecular basis for pod spine existence in okra.

 

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