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RAPID MULTIPLICATION OF ORNAMENTAL BULBOUS PLANTS OF Lilium orientalis
AND Lilium longiflorum
Shumaila Khan1*,
Muhammad Jafar Jaskani1, M.
Zafar Iqbal2 and Ashifa Rafiq1
1Institute
of Horticultural Sciences, University of Agriculture, Faisalabad,
Pakistan
2Department
of Plant Pathology, Bahauddin Zakariya university, Multan, Pakistan
*Corresponding author (e-mail:
shailadimple@hotmail.com)
Abstract
Lilium is one of the most important cut flowers all over the world. It
ranks within the ten top most beautiful cut flowers because of its
attractive and large showy flowers. Ornamental lily hybrids have been
developed in very large amount. Effect of different growth hormones and
different growth media was observed in this study. Bulb scales were used
as an explant, cultured in vitro on Murashige and Skoog (MS)
medium containing different concentrations of 6-benzyladenine (BA) and
naphthalene acetic acid (NAA). Among different treatments used for plant
culturing, the MS medium supplemented with 6-benzylaminopurine (BAP) 3.0
mg/L was found to be the best for shoot initiation from scales of bulbs.
After that plants were transferred to different media for multiple
shooting. Out of different concentrations used the medium with 1.0 mg/L
BAP and 1.0 mg/L NAA increased frequency of shoot formation up to 90%.
An average of about 10 ± 3.73 roots/explants with well-developed roots
and bulblet formation were obtained in this medium.
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